P-87: Effects of Bovine Cumulus Cell Co-Cul tire on Post Fertilization Development of Buffalo (Bubalus bubalis) Oocyte
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Abstract:
Background: There is a considerable numbers of buffalo (Bubalus bubalis) in Iran which have an important role in rural life. The reproductive performance of buffalo is lower than cow. Co-culture with somatice cells improved the maturation rate of most of the species. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on post fertilization development of buffalo oocyte. Materials and Methods: Ovaries were collected from local slaughterhouse. The cumulus oocyte complexes (COC) were picked up form follicles (2-8 mm). The grade A and B COCs were randomly divided into 3 groups. Group 1: COCs were subjected to oocyte maturation medium containing TCM-199+ 5%FCS + 5%FF + 10mg/ml LH + 10 mg/ml FSH. Group 2: the COCs were cultured on a monolayer culture of bovine cumulus cells in a medium contained TCM199 with 5% FCS. Group 3: the denuded COCs were subjected on a monolayer culture of bovine cumulus cells in a medium similar to group 2. After 24 hours of maturation in an atmosphere with 5% CO2 and 95% relative humidity, oocytes subjected to in vitro fertilization using buffalo epididymal sperm. Three and six days after insemination (in BO medium) cleavage and blastocyst rates were evaluated. Results: Mean percentage of embryos cleaved for each were 41.3, 48.2 and 32.9 in groups 1, 2 and 3 (p>0.05), respectively. There was no developed embryo to blastocyst in all groups. Conclusion: The bovine cumulus co-culture with buffalo oocytes was effective as a completed in developmental potential of buffalo oocytes.
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Journal title
volume 6 issue 2
pages -
publication date 2012-09-01
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